Prevalence sample‐based data should be transmitted from Member States to the European Food Safety Authority (EFSA) using the EFSA Standard Sample Description version 2 (SSD2) standard. To support reporting countries in data transmission using eXtensible Markup Language (XML) data transfer, specific guidance is given in this report covering the reporting of sample‐based zoonoses and zoonotic agent data. This guidance is specifically aimed at guiding the reporting of information under the framework of Directive 2003/99/EC, Regulation (EU) 2017/625, Commission Implementing Regulation (EU) 2019/627 and of Commission Delegated Regulation (EU) 2018/772. The objective is to explain in detail the individual data elements that are included in the EFSA (SSD2) data model to be used for the XML prevalence sample‐based data transmission through the Data Collection Framework according to the protocol described in the EFSA Guidance on Data Exchange version 2 (GD2). In particular, data elements to be reported are explained, including information about the data type, a reference to the list of allowed terms and business rules or requirements that may apply.