The European Food Safety Authority (EFSA) carried out a public consultation to receive input from the scientific community and all interested parties on the update of the Guidance on the assessment of the toxigenic potential of Bacillus species used in animal nutrition. The draft Guidance was prepared by the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP Panel) and endorsed for public consultation at its Plenary meeting on 18 June 2013. The written public consultation for this document was open from 26 June to 30 September 2013. EFSA received comments from 12 interested parties. EFSA and its FEEDAP Panel wish to thank all stakeholders for their contributions. The current report summarises the outcome of the public consultation and includes a brief summary of the comments received and how these were addressed. The FEEDAP Panel prepared an updated version of the Guidance on the assessment of the toxigenic potential of Bacillus species used in animal nutrition taking into account the questions/comments received. This guidance was discussed and adopted at the FEEDAP Plenary meeting on 8 April 2014, and is published in the EFSA Journal.