The European Food Safety Authority (EFSA) carried out a public consultation to receive input from the scientific community and all interested parties on the draft guidance on the characterisation of microorganisms used as feed additives or as production organisms prepared by the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) and endorsed by the Panel for public consultation at its Plenary meeting on 18 May 2017.1 The written public consultation for this document was open from 15 June to 15 September 2017. EFSA received comments from 15 interested parties. EFSA and its FEEDAP Panel wish to thank all stakeholders for their contributions. The current report summarises the outcome of the public consultation, and includes the comments received and how the comments were addressed. The FEEDAP Panel prepared an updated version of the guidance on the characterisation of microorganisms used as feed additives or as production organisms taking into account the questions/comments received. This guidance was discussed and adopted at the FEEDAP Plenary meeting on 21 February 2018, and is published in the EFSA Journal.