This report presents the outcome of extensive literature search on studies related to the chemistry and toxicity of glycoalkaloids and quinolizidine alkaloids in food and feed. The systematic literature search captures available scientific information published since 1950. 3,147 references were identified as potentially related to the chemistry and toxicity of glycoalkaloids in food and feed and 8,287 as potentially related to the chemistry and toxicity of quinolizidine alkaloids in food and feed. Upon screening of titles and abstracts of the candidates concerning glycoalkaloids, 1,645 were regarded as relevant, the number of included potentially relevant studies concerning quinolizidine alkaloids was 1,783. All included studies were characterised according to 10 different areas of interest of EFSA for further investigation.