The European Food Safety Authority (EFSA) carried out a public consultation to receive input from the scientific community and all interested parties on the draft Scientific Opinion on Dietary Reference Values (DRVs) for choline, prepared by the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA Panel) and endorsed by the Panel for public consultation at its Plenary meeting on 2 February 2016. The written public consultation for this document was open from 22 February 2016 to 21 March 2016. EFSA received comments from three interested parties. EFSA and its NDA Panel wish to thank all stakeholders for their contributions. The current report summarises the outcome of the public consultation, and includes a brief summary of the comments received and how the comments were addressed. The NDA Panel prepared an updated version of the Scientific Opinion on DRVs for choline taking into account the comments received. The Scientific Opinion was discussed and adopted at the NDA Plenary meeting on 21 April 2016, and is published in the EFSA Journal.