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Scientific Opinion on the safety and efficacy of Bacillus amyloliquefaciens (NCIMB 30229) as a silage feed additive for all species

EFSA Journal 2013;11(1):3042 [11 pp.]. doi:10.2903/j.efsa.2013.3042
  EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) Panel Members Gabriele Aquilina, Alex Bach, Vasileios Bampidis, Maria De Lourdes Bastos, Gerhard Flachowsky, Josep Gasa-Gasó, Mikolaj Antoni Gralak, Christer Hogstrand, Lubomir Leng, Secundino López-Puente, Giovanna Martelli, Baltasar Mayo, Derek Renshaw, Guido Rychen, Maria Saarela, Kristen Sejrsen, Patrick Van Beelen, Robert John Wallace and Johannes Westendorf. Acknowledgment The Panel wishes to thank the members of the Working Group on Silage, including Andrew Chesson, Pier Sandro Cocconcelli, Jürgen Gropp and Miklós Mézes, for the preparatory work on this scientific opinion. Contact FEEDAP@efsa.europa.eu
Type: Opinion of the Scientific Committee/Scientific Panel On request from: European Commission Question number: EFSA-Q-2011-00389 Adopted: 11 December 2012 Published: 11 January 2013 Affiliation: European Food Safety Authority (EFSA), Parma, Italy
Abstract

A strain of Bacillus amyloliquefaciens is intended to be added to forages to promote the ensiling process (technological additive, functional group: silage additive). The identity of the strain and its susceptibility to relevant antibiotics has been established. However, the strain produces cyclic lipopeptides which possess potent surfactant activity, known to be involved in food intoxication. Although the strain is intended for use only in the production of silage, as a spore former it will survive the ensiling process and be ingested by target animals. The spores will also survive passage through the gastrointestinal tract of animals and be a potential source of contamination of food of animal origin and of the environment. The greatest risk would be to those handling the product on farm following oral, dermal and respiratory exposure. Consequently, the FEEDAP Panel concludes that use of the strain in the production of silage presents a hazard to consumers, users and the environment, and potentially also to the target animals. A total of three laboratory experiments with laboratory-scale silos, each lasting at least 90 days, were carried out using samples of forage of differing dry matter and water-soluble carbohydrate content. In each case, replicate silos containing treated forage were compared with identical silos containing the same but untreated forage. The potential of the additive to improve aerobic stability of silages at the proposed dose of 5.0 x 107 CFU/kg fresh materials was not convincingly demonstrated.

© European Food Safety Authority, 2013

Summary

Following a request from the European Commission, the Panel on Additives, Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety for the target animals, consumer, user and the environment and on the efficacy of a product based on a single strain of Bacillus amyloliquefaciens, when used as a technological additive intended to improve the ensiling process at a proposed dose of 5 x 107 CFU/kg fresh material of easy and moderately difficult to ensile forages.

The identity of B. amyloliquefaciens NCIMB 30229 and its susceptibility to relevant antibiotics has been established. However, the strain produces cyclic lipopeptides which possess potent surfactant activity, known to be involved in food intoxication. Although the strain is intended for use only in the production of silage, as a spore former it will survive the ensiling process and be ingested by target animals. The spores will also survive passage through the gastrointestinal tract of animals and be a potential source of contamination of food of animal origin and of the environment. The greatest risk would be to those handling the product on farm. Consequently, the FEEDAP Panel concludes that use of the strain in the production of silage presents a hazard to consumers, users and the environment, and potentially also to the target animals.

A total of three experiments with laboratory-scale silos, each lasting at least 90 days, were carried out using samples of forage of differing dry matter and water-soluble carbohydrate content. In each case, replicate silos containing treated forage were compared with identical silos containing the same but untreated forage. The potential of the additive to improve aerobic stability of silages at the proposed dose of 5.0 x 107 CFU/kg fresh material was not convincingly demonstrated.
 

Keywords

technological additive, silage additive, Bacillus amyloliquefaciens, QPS, safety, efficacy