Analysis of the baseline survey on the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in holdings with breeding pigs, in the EU, 2008 - Part B: factors associated with MRSA contamination of holdings


European Food Safety Authority

EFSA wishes to thank the members of the Task Force on Zoonoses Data Collection that reviewed and endorsed this report: Andrea Ammon, Alenka Babusek, Lisa Barco, Marta Bedriova, Susan Chircop, Marianne Chriel, Georgi Chobanov, Ingrid Dan, Jürg Danuser, Noel Demicoli, Kris De Smet, Sylvie Francart, Matthias Hartung, Birgitte Helwigh, Merete Hofshagen, Patrícia Inácio, Sarolta Idei, Elina Lahti, Lesley Larkin, Peter Much, Edith Nagy, Lisa O’Connor, Rob Van Oosterom, Jacek Osek, José Luis Saez Llorente, Antonio Petrini, Melanie Picherot, Christodoulos Pipis, Saara Raulo, Petr Šatrán, Joseph Schon, Jelena Sõgel, Snieguole Sceponaviciene, Ana María Troncoso González, Kilian Unger, Luc Vanholme, Dimitris Vourvidis, Nicole Werner-Keišs. The contribution of the members of the working group that prepared this scientific report is gratefully acknowledged: Antonio Battisti, Hanne-Dorthe Emborg, Concepción Porrero, Pascal Sanders, Gertraud Schuepbach, Christopher Teale, Bernd-Alois Tenhagen, and Jaap Wagenaar; and that of EFSA’s contractors: Hendriek Boshuizen from National Institute for Public Health and Environment (RIVM); Frank Aarestrup, Henrik Hasman, and Antonio Vieira from Danish Technical University, National Food Institute, as well as EFSA’s staff members: Pierre-Alexandre Belœil, Giusi Amore, Francesca Riolo, and Frank Boelaert for the support provided to this EFSA scientific output.

EFSA Journal
EFSA Journal 2010; 8(6):1597 [67 pp.].
Scientific Report of EFSA
On request from
European Commission
Question Number
10 May 2010
3 June 2010
European Food Safety Authority (EFSA), Parma, Italy

A European Union-wide baseline survey on methicillin-resistant Staphylococcus aureus (MRSA) was conducted in 2008 in holdings with breeding pigs. A total of 1,600 holdings housing and selling mainly breeding pigs (breeding holdings), and 3,473 holdings housing breeding pigs and selling mainly pigs for fattening or slaughter (production holdings) from 24 Member States and two other European countries were included in the survey. Pooled dust samples collected from holdings were tested for MRSA. The likelihood of a limited set of holding-level factors to be potentially associated with MRSA-positive holdings were analysed by multiple regression analysis, separately for breeding and for production holdings. These analyses showed that the risk of holdings of being contaminated with MRSA increased as the number of breeding pigs in the holding increased, in both breeding and production holdings. Analyses at country-level demonstrated a strong positive association between the prevalence of MRSA-positive breeding holdings and MRSA-positive production holdings, suggesting a vertical dissemination of MRSA between the holdings. A complementary country-level analysis using TRAde Control and Expert System data on intra-Community trade further disclosed strong positive associations between the prevalence of MRSA-positive holdings with breeding pigs and the volume of imported breeding pigs, and a proxy for numbers of imported breeding pigs at risk of being colonised with MRSA. Further investigation of the diversity of MRSA Staphylococcus protein A types (spa-types) showed that the distribution of spa-types differed significantly between countries. Spa-types belonging to lineages MRSA ST1, ST5, and ST8 tested negative for Panton-Valentine leukocidin (PVL) toxin genes, suggesting that these strains were not related to the PVL-positive MRSA strains known in human medicine. Further investigations of risk factors for MRSA holding contamination at country level, as well as investigations on prevention and intervention measures to contain MRSA contamination in pig holdings are recommended.

MRSA, spa-typing, pigs, breeding pigs, baseline survey, risk factors, EU
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