Analysis of the baseline survey on the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses in the EU, 2008 - Part A: Campylobacter and Salmonella prevalence estimates

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Article
European Food Safety Authority
EFSA Journal
EFSA Journal 2010; 8(03):1503 [100 pp.].
doi
10.2903/j.efsa.2010.1503
Acknowledgements

EFSA wishes to thank the members of the Task Force on Zoonoses Data Collection that endorsed this report: Andrea Ammon, Alenka Babusek, Lisa Barco, Marta Bedriova, Susan Chircop, Marianne Chriel, Georgi Chobanov, Ingrid Dan, Jürg Danuser, Noel Demicoli, Kris De Smet, Sylvie Francart, Matthias Hartung, Birgitte Helwigh, Merete Hofshagen, Patrícia Inácio, Sarolta Idei, Elina Lahti, Lesley Larkin, Peter Much, Edith Nagy, Lisa O’Connor, Rob Van Oosterom, Jacek Osek, José Luis Paramio Lucas, Antonio Petrini, Melanie Picherot, Christodoulos Pipis, Saara Raulo, Petr Šatrán, Joseph Schon, Jelena Sõgel, Snieguole Sceponaviciene, Ana María Troncoso González, Kilian Unger, Luc Vanholme, Dimitris Vourvidis, Nicole Werner-Keišs. The contribution of the members of the working group that prepared this scientific report is gratefully acknowledged: Dirk Berkvens, Enne de Boer, Marjaana Hakkinen, Mary Howell, Annemarie Kaesbohrer, Micheál O’Mahony, Eva Olsson Engvall, Hanne Rosenquist, Moez Sanaa, Mieke Uyttendaele, Arjen van de Giessen and Jarp Wagenaar; and that of EFSA’s contractors: Lieven De Zutter from University of Ghent; Tine Hald from Danish Technical University, National Food Institute, Saskia Litière and Marc Aerts from University of Hasselt, and Alessandro Mannelli from University of Turin, as well as EFSA’s staff members: Maria Teresa Da Silva Felício, Kenneth Mulligan, and Frank Boelaert for the support provided to this EFSA scientific output.

Type
Scientific Report of EFSA
On request from
European Commission
Question Number
EFSA-Q-2008-416A
Approved
31 January 2010
Published
17 March 2010
Last Updated
16 September 2011. This version replaces the previous one/s.
Affiliation
European Food Safety Authority (EFSA), Parma, Italy
Note
Download Article (1.36 MB)
Abstract

A European Union-wide baseline survey on Campylobacter in broiler batches and on Campylobacter and Salmonella on broiler carcasses was carried out in 2008. A total of 10,132 broiler batches were sampled from 561 slaughterhouses in 26 European Union Member States and two countries not belonging to the European Union. From each randomly selected batch the caecal contents of 10 slaughtered broilers were collected, pooled and examined for Campylobacter. From the same batch one carcass was collected after chilling and the neck skin together with the breast skin was examined for the presence of Campylobacter and Salmonella, in addition to the determination of the Campylobacter counts. Campylobacter was detected in pooled caecal contents of broilers and on broiler carcasses in all participating countries. At Community level the prevalence of Campylobacter-colonised broiler batches was 71.2% and that of Campylobacter-contaminated broiler carcasses was 75.8%. The Member State prevalence varied from 2.0% to 100.0% and from 4.9% to 100.0%, for caecal contents and carcasses, respectively. The results of the counts of Campylobacter on broiler carcasses showed substantial variation among the countries in contamination levels. About two-thirds of the Campylobacter isolates from the pooled caecal contents as well as from the broiler carcasses were identified as Campylobacter jejuni, while one-third was Campylobacter coli. Twenty-two Member States and one non-Member State isolated Salmonella on the broiler carcasses, with a Community prevalence of 15.6%. This prevalence varied widely among the Member States, from 0.0% to 26.6%. However, one Member State had an exceptionally high prevalence of 85.6% with the majority of isolates being S. Infantis. The Community prevalence of Salmonella Enteritidis or Salmonella Typhimurium-contaminated broiler carcasses was 3.6%. Salmonella Infantis and Salmonella Enteritidis were the two most frequently isolated serovars on broiler carcasses in the EU and accounted for about one-third and one-sixth of the Salmonella isolates, respectively.

Summary

In the European Union, campylobacteriosis and salmonellosis are the two most frequently reported food-borne illnesses in humans. Broiler meat is considered to be an important food-borne source of both these human diseases.

In order to establish baseline and comparable values for all Member States, a European Union-wide baseline survey was carried out at slaughterhouse level to determine the prevalence of Campylobacter in broiler batches and of Campylobacter and Salmonella on broiler carcasses. The broiler batches and carcasses were randomly selected from the broiler slaughterhouses within each Member State. This was the sixth baseline survey to be conducted in the European Community and it was the first baseline survey directly investigating foodstuffs.

Sampling took place between January and December 2008. A total of 10,132 broiler batches sampled from 561 slaughterhouses in 26 European Union Member States, plus Norway and Switzerland, were included in the survey. From each selected batch the caecal contents of 10 slaughtered broilers were collected, pooled and examined for Campylobacter. Furthermore, from the same batch one carcass was collected immediately after chilling and the neck skin together with the breast skin was examined for the presence of Campylobacter and Salmonella, in addition to the determination of the Campylobacter counts. At least one Campylobacter isolate was speciated from each positive sample and also at least one isolate serotyped from each Salmonella-positive sample.

Campylobacter was detected in pooled caecal contents of broilers and on broiler carcasses in all 26 participating Member States and the two non-Member States. At Community level the prevalence of Campylobacter-colonised broiler batches was 71.2% and that of Campylobacter-contaminated broiler carcasses was 75.8%. Member State prevalence varied from 2.0% to 100.0% and from 4.9% to 100.0%, for caecal contents and carcasses, respectively. About two-thirds of the Campylobacter isolates from the broiler batches as well as those from the broiler carcasses were identified as Campylobacter jejuni, while one-third was Campylobacter coli. Few were speciated as other Campylobacter species.

The counts of Campylobacter bacteria on broiler carcasses varied also widely between countries. In general there was a tendency for high counts in countries with high Campylobacter prevalence. In the European Union, almost half (47.0%) of the carcasses contained less than 10 Campylobacter per g (cfu/g) and 12.2% contained between 10-99 cfu/g. Higher counts were detected as follows: between 100-999 cfu/g on 19.3%, between 1,000-10,000 cfu/g on 15.8% and more than 10,000 cfu/g on 5.8% of carcasses.

Twenty-two of the 26 participating Member States and one non-Member State isolated Salmonella from the broiler carcass samples, with a Community prevalence of Salmonella-contaminated broiler carcasses of 15.6% at slaughterhouse level. The prevalence of Salmonella-contaminated broiler carcasses varied widely among Member States, from 0.0% to 26.6%. However, Hungary had an exceptionally high prevalence of 85.6% with the majority of isolates being Salmonella Infantis. Seventeen Member States isolated Salmonella Enteritidis or Typhimurium. This resulted in an estimated Community prevalence of Salmonella Enteritidis or Salmonella Typhimurium-contaminated broiler carcasses of 3.6%, varying from 0.0% to 9.6% within Member States.

At European Union level the four most frequently isolated Salmonella serovars on broiler carcasses were respectively, in decreasing order, Salmonella Infantis (29.2% of the Salmonella positive broiler carcass samples), Salmonella Enteritidis (13.6%), Salmonella Kentucky (6.2%) and Salmonella Typhimurium (4.4%). Out of these Salmonella Enteritidis and Salmonella Typhimurium are commonly reported in human salmonellosis cases in the European Union, whereas the Salmonella Infantis and Salmonella Kentucky generally constitute a minor proportion of human infections. Seventy-five percent of the Salmonella Infantis-positive samples were reported by Hungary. The serovar distribution varied among Member States, many of them having a specific distribution pattern.

The Member State and European Union level prevalence presented in the report are apparent prevalences, meaning that the prevalence estimates do not account for imperfect test characteristics.

Broiler meat is considered an important food-borne source of both human Campylobacter and Salmonella infections in the European Union. The risk for human health arises from consumption of under-cooked the meat or cross-contamination of other foods. Safe handling of raw meat, thorough cooking and strict kitchen hygiene should prevent or reduce the risk posed by Campylobacter and Salmonella-contaminated broiler meat.

The Campylobacter and Salmonella baseline figures may be used in the future to follow trends and to evaluate the impact of control and monitoring programmes. The figures also provide useful information for setting reduction and performance objectives and possibly for evaluating some potential intervention methods. However, further research on the epidemiology and surveillance methods of Campylobacter in the broiler meat production is recommended. In the national Salmonella control and surveillance programmes of broiler flocks and broiler meat, Member States may need to address serovars other than Salmonella Enteritidis and Salmonella Typhimurium.

Keywords
Campylobacter, Salmonella, broiler batches, broiler carcasses, chicken, survey, prevalence, EU
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Number of Pages
100